Validation of Array-Based Gene Expression Profiles by Real-Time (Kinetic) RT-PCR
نویسندگان
چکیده
منابع مشابه
Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR.
We evaluated real-time (kinetic) reverse transcription-polymerase chain reaction (RT-PCR) to validate differentially expressed genes identified by DNA arrays. Gene expression of two keratinocyte subclones differing in the physical state of human papillomavirus (episomal or integrated) was used as a model system. High-density filter arrays identified 444 of 588 genes as either negative or expres...
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The effects of serum on the expression of four commonly used housekeeping genes were examined in serum-stimulated fibroblasts in order to validate the internal control genes for a quantitative RT-PCR assay. NIH 3T3 fibroblasts transfected with an inducible chimeric gene were serum-starved for 24 h and then induced with 15% serum for 8 h. Serum did not alter the amount of total RNA that was expr...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2001
ISSN: 1525-1578
DOI: 10.1016/s1525-1578(10)60646-0